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pTA-attB
(Plasmid #18937)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18937 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCR2.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3805
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phiC31 attB
  • Alt name
    phiC31 bacterial attachment site
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    285
  • GenBank ID
    X60952

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTA-attB was a gift from Michele Calos (Addgene plasmid # 18937 ; http://n2t.net/addgene:18937 ; RRID:Addgene_18937)
  • For your References section:

    A phage integrase directs efficient site-specific integration in human cells. Groth AC, Olivares EC, Thyagarajan B, Calos MP. Proc Natl Acad Sci U S A. 2000 May 23. 97(11):5995-6000. 10.1073/pnas.090527097 PubMed 10801973