Addgene - pUASTB Plasmid Data

Plasmid 18944: pUASTB

Gene/insert name:
phiC31 attB
Alt name:
phiC31 bacterial attachment site
Insert size:
279
Species:
S. cerevisiae (budding yeast)
GenBank ID:
X60952
Vector backbone:
pUAST
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
7804
Cloning site 5':
BamHI
Site destroyed during cloning:
Yes
Cloning site 3':
BamHI
Site destroyed during cloning:
Yes
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Michele Calos
Terms and Licenses:
MTA

Comments:

AttB residues 8033-228

mini-white residues 229-4384

P Element residues 7556-7962,
4385-4976

Amp residues 5987-6829

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Groth et al (Genetics. 2004 Apr . 166(4):1775-82. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18944" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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