pJL44 - Phsp16.48::MosTase::glh-2utr
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19333||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2530
Vector typeWorm Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePhsp-16::Mos1 transposase
SpeciesC. elegans (nematode)
Insert Size (bp)2279
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
This plasmid was made by Dr. Jean Louis Bessereau and described in the publication: Bessereau JL, Wright A, Williams DC, Schuske K, Davis MW, Jorgensen EM.
Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line.
Nature. 2001 Sep 6;413(6851):70-4.
There is che-1 sequence in the plasmid. It comes from the glh-2 3' UTR fragment in the plasmid. The UTR fragment extends into the 3' region of the che-1 gene, which is tail to tail with glh-2 on the chromosome. This is only the last exon of che-1 and it is not relevant to the function of the construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJL44 - Phsp16.48::MosTase::glh-2utr was a gift from Erik Jorgensen (Addgene plasmid # 19333 ; http://n2t.net/addgene:19333 ; RRID:Addgene_19333)
For your References section:Single-copy insertion of transgenes in Caenorhabditis elegans. Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, Blake J Newman, Jason M Thumme, Søren-Peter Olesen, Morten Grunnet & Erik M Jorgensen. 10.1038/ng.248 PubMed 18953339