Addgene - MSCV FLIPi P2G_Thy1.1 PTEN Plasmid Data

Plasmid 19749: MSCV FLIPi P2G_Thy1.1 PTEN

Gene/insert name:
oligo targeting PTEN
Alt name:
PTEN
Insert size:
387
Species:
M. musculus (mouse)
Entrez Gene:
Pten (2310035O07Rik, A130070J02Rik, AI463227, B430203M17Rik, MMAC1, TEP1)
Vector backbone:
MSCV FLIPi
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral, RNAi, Cre/Lox
Backbone size w/o insert (bp):
7994
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
PmeI
Site destroyed during cloning:
No
5' sequencing primer:
GFP miR primer (GCTGGAGTTCGTGACCGCC) List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
Growth instructions:
grow in stbl3 cells at 30oC
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Supplemental document:
FLIP vector protocols (application/pdf)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Richard Hynes
Terms and Licenses:
MTA

Comments:

Constitutively expresses puromycin-resistance and GFP. Recombines to express surface Thy1.1 and miR30-based RNAi. The miRNA is contained within an artificial intron, and splicing of the intron can boost transgene expression (1.5 to 2-fold). The expression of surface Thy1.1 facilitates antibody-mediated cell sorting.

See FLIP vector protocols (PDF from this page) for more information.

There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A system for Cre-regulated RNA interference in vivo. Stern et al (Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19749" in your Materials and Methods section.