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Plasmid 19744: MSCV FLIP FF
  • oligo targeting Firefly luciferase

  • 386

  • MSCV FLIP
    (Search Vector Database)

  • Mammalian Expression, Retroviral, RNAi, Cre/Lox

  • 7926

  • NotI

  • No

  • PmeI

  • No

  • GFP miR primer (GCTGGAGTTCGTGACCGCC) List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 30

  • grow in stbl3 cells at 30oC

  • Unknown

  • Puromycin

  • View sequences (2)
  • View map

  • FLIP vector protocols (application/pdf)

  • Richard Hynes

  • UBMTA
    Luciferase Limited Use Label License

Comments: 

Constitutively expresses puromycin-resistance and the surface molecule Thy1.1. Recombines to express GFP and miR30-based RNAi.

There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
This is the control vector, which can be used for cloning. This vector expresses Firefly luciferase.

See FLIP vector protocols (PDF from this page) for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A system for Cre-regulated RNA interference in vivo. Stern et al (Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19744" in your Materials and Methods section.