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MSCV FLIP FF
(Plasmid #19744)

Backbone

  • Vector backbone
    MSCV FLIP
  • Backbone size w/o insert (bp) 7926
  • Vector type
    Mammalian Expression, Retroviral, RNAi, Cre/Lox
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    grow in stbl3 cells at 30oC
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    oligo targeting Firefly luciferase
  • Insert Size (bp)
    386

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer GFP miR primer (GCTGGAGTTCGTGACCGCC)
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Constitutively expresses puromycin-resistance and the surface molecule Thy1.1. Recombines to express GFP and miR30-based RNAi.

There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
This is the control vector, which can be used for cloning. This vector expresses Firefly luciferase.

See FLIP vector protocols (PDF from this page) for more information.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV FLIP FF was a gift from Richard Hynes (Addgene plasmid # 19744)
  • For your References section:

    A system for Cre-regulated RNA interference in vivo. Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO. Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900. 10.1073/pnas.0806907105 PubMed 18779577