MSCV FLIP FF
Vector backboneMSCV FLIP
- Backbone size w/o insert (bp) 7926
Vector typeMammalian Expression, Retroviral, RNAi, Cre/Lox
Growth in Bacteria
Growth instructionsgrow in stbl3 cells at 30oC
Gene/Insert nameoligo targeting Firefly luciferase
Insert Size (bp)386
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site PmeI (not destroyed)
- 5′ sequencing primer GFP miR primer (GCTGGAGTTCGTGACCGCC) (Common Sequencing Primers)
Constitutively expresses puromycin-resistance and the surface molecule Thy1.1. Recombines to express GFP and miR30-based RNAi.
There are minor discrepancies between the depositor's sequence and Addgene's sequencing results. The mismatches are either coding joints or a sequencing difference between the actual and the published miR30 vector sequence.
This is the control vector, which can be used for cloning. This vector expresses Firefly luciferase.
See FLIP vector protocols (PDF from this page) for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV FLIP FF was a gift from Richard Hynes (Addgene plasmid # 19744)
For your References section:A system for Cre-regulated RNA interference in vivo. Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO. Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900. 10.1073/pnas.0806907105 PubMed 18779577
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.