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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20020 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGL2B
- Backbone size w/o insert (bp) 5598
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIl12b promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)405
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Entrez GeneIl12b (a.k.a. Il-12b, Il-12p40, Il12p40, p40)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site BglII (unknown if destroyed)
- 5′ sequencing primer GLprimer1
- 3′ sequencing primer GLprimer2 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Luciferase Reporter driven by 400 base pair promoter of the Il12b gene.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Il12b 400bp pro-Luc was a gift from Stephen Smale (Addgene plasmid # 20020 ; http://n2t.net/addgene:20020 ; RRID:Addgene_20020) -
For your References section:
Multiple control elements mediate activation of the murine and human interleukin 12 p40 promoters: evidence of functional synergy between C/EBP and Rel proteins. Plevy SE, Gemberling JH, Hsu S, Dorner AJ, Smale ST. Mol Cell Biol. 1997 Aug . 17(8):4572-88. PubMed 9234715
