Il12b 400bp pro-Luc
(Plasmid #20020)

• Depositing Lab: Stephen Smale
• Publication: Plevy et al Mol Cell Biol. 1997 Aug . 17(8):4572-88.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	20020	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pGL2B
  (Search Vector Database)
• Backbone size w/o insert (bp): 5598
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Il12b promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 405
• Entrez Gene: Il12b (a.k.a. Il-1, Il-12, Il-12b, Il-12p40, Il12p40, p40)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: BglII (unknown if destroyed)
• 5′ sequencing primer: GLprimer1
• 3′ sequencing primer: GLprimer2

Resource Information

• Terms and Licenses:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 2 References

Depositor Comments

Luciferase Reporter driven by 400 base pair promoter of the Il12b gene.