pCAGMKOSiE
(Plasmid #20865)

• Depositing Lab: Keisuke Kaji
• Publication: Kaji et al Nature. 2009 Mar 1. ():.

Backbone

• Vector backbone: pCAG-ires-EGFP
• Backbone size w/o insert (bp): 6397
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5a
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene
• Alt name: c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2
• Species: M. musculus (mouse)
• Insert Size (bp): 4971
• GenBank ID: BC006728 NM_011443 NM_013633 NM_010637
• Entrez Gene: Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry