Addgene - pGL-Lats2UTR Plasmid Data

Plasmid 20882: pGL-Lats2UTR

Gene/insert name:
Lats2 3'UTR
Insert size:
1600
Species:
M. musculus (mouse)
GenBank ID:
NM_015771
Vector backbone:
pGL3-Control
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
5256
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
GGAAAACTCGACGCAAGA List of Sequencing Primers
3' sequencing primer:
CACATTTGTAGAGGTTTTACTTGCT
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Robert Blelloch
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

A C->T mutation at nt530 in Addgene's sequence should not affect the function of this plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Embryonic stem cell-specific microRNAs regulate the G1-S transition and promote rapid proliferation. Wang et al (Nat Genet. 2008 Dec . 40(12):1478-83. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20882" in your Materials and Methods section.