Addgene - PBS/pU6-HIF1 alpha RNAi plasmid 2 Plasmid Data

Plasmid 21104: PBS/pU6-HIF1 alpha RNAi plasmid 2

Gene/insert name:
RNAi against hypoxia inducible factor 1alpha
Insert size:
70
Species:
H. sapiens (human), M. musculus (mouse)
Vector backbone:
PBS/U6
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3300
Cloning site 5':
APAI
Site destroyed during cloning:
No
Cloning site 3':
ECORI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Connie Cepko
Terms and Licenses:
MTA

Comments:

HIF1α RNAi target sequence:
GGGTTGAAACTCAAGCAACTG.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: HDAC4 regulates neuronal survival in normal and diseased retinas. Chen et al (Science. 2009 Jan 9. 323(5911):256-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21104" in your Materials and Methods section.