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PBS/pU6-HIF1 alpha RNAi plasmid 2
(Plasmid #21104)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    RNAi against hypoxia inducible factor 1alpha
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    70

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site APAI (not destroyed)
  • 3′ cloning site ECORI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

HIF1α RNAi target sequence:
GGGTTGAAACTCAAGCAACTG.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PBS/pU6-HIF1 alpha RNAi plasmid 2 was a gift from Connie Cepko (Addgene plasmid # 21104)
  • For your References section:

    HDAC4 regulates neuronal survival in normal and diseased retinas. Chen B, Cepko CL. Science. 2009 Jan 9. 323(5911):256-9. 10.1126/science.1166226 PubMed 19131628