PBS/pU6-HIF1 alpha RNAi plasmid 2
- Backbone size w/o insert (bp) 3300
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
Gene/Insert nameRNAi against hypoxia inducible factor 1alpha
SpeciesH. sapiens (human), M. musculus (mouse)
Insert Size (bp)70
- Cloning method Restriction Enzyme
- 5′ cloning site APAI (not destroyed)
- 3′ cloning site ECORI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
HIF1α RNAi target sequence:
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PBS/pU6-HIF1 alpha RNAi plasmid 2 was a gift from Connie Cepko (Addgene plasmid # 21104)
For your References section:HDAC4 regulates neuronal survival in normal and diseased retinas. Chen B, Cepko CL. Science. 2009 Jan 9. 323(5911):256-9. 10.1126/science.1166226 PubMed 19131628
Map generated by Addgene from plasmid data.