PBS/pU6-HIF1 alpha RNAi plasmid 2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21104||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3300
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRNAi against hypoxia inducible factor 1alpha
SpeciesH. sapiens (human), M. musculus (mouse)
Insert Size (bp)70
- Cloning method Restriction Enzyme
- 5′ cloning site APAI (not destroyed)
- 3′ cloning site ECORI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
HIF1α RNAi target sequence:
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PBS/pU6-HIF1 alpha RNAi plasmid 2 was a gift from Connie Cepko (Addgene plasmid # 21104)
For your References section:HDAC4 regulates neuronal survival in normal and diseased retinas. Chen B, Cepko CL. Science. 2009 Jan 9. 323(5911):256-9. 10.1126/science.1166226 PubMed 19131628
Generated by Addgene from plasmid data.