Addgene - pBABE-puro-mouse Oct4 Plasmid Data

Plasmid 21110: pBABE-puro-mouse Oct4

Gene/insert name:
Oct4
Alt name:
Pou5f1
Insert size:
1059
Species:
M. musculus (mouse)
Entrez Gene:
Pou5f1 (NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
Vector backbone:
pBABE-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
EcoR1
Site destroyed during cloning:
No
Cloning site 3':
EcoR1
Site destroyed during cloning:
No
5' sequencing primer:
gcctcaatcctccctttatcc List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Dean Tantin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A general mechanism for transcription regulation by Oct1 and Oct4 in response to genotoxic and oxidative stress. Kang et al (Genes Dev. 2009 Jan 15. 23(2):208-22. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21110" in your Materials and Methods section.