pBigT
(Plasmid #21270)

• Purpose: (Empty Backbone) Used for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.
• Depositing Lab: Frank Costantini
• Publication: Srinivas et al BMC Dev Biol. 2001 . 1():4.

Backbone

• Vector backbone: pBigT
• Backbone manufacturer: Costantini Lab
• Backbone size (bp): 6117
• Vector type: Mouse Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: M13-fwd
• 3′ sequencing primer: M13-rev

Resource Information

• Supplemental Documents:
  • Annotated Genbank file
• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBigT is used for making transgenic mice. The plasmid contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence. See article for detailed information regarding the creation and use of this plasmid.

Information for Cloning Grade DNA (Catalog # 21270-DNA.cg) ( Back to top )

Item is discontinued.

Addgene no longer distributes this item. Contact for more information.

2 µg of cloning grade DNA in Tris buffer

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here