Addgene - SV40 Large T K1 mutant in pENTR1A (w414-1) Plasmid Data

Plasmid 22270: SV40 Large T K1 mutant in pENTR1A (w414-1)

Gene/insert name:
SV40 Large T antigen TK1 mutant (E107K)
Alt name:
TK1
Insert size:
2126
Mutation:
E107K (K1 mutation)
Vector backbone:
pENTR1A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Entry vector
Backbone size w/o insert (bp):
2279
Cloning site 5':
Bam HI
Site destroyed during cloning:
Yes
Cloning site 3':
Xho I
Site destroyed during cloning:
Yes
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Large T K1 was obtained from Dr. Francis Rodier.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

Large T antigen K1 mutant can no longer bind and inactivate the Rb, p130 and p107 proteins (DeCaprio et al. 1988, Stubdal et al. 1997). It also fails to activate Akt (Yu and Alwine 2008).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22270" in your Materials and Methods section.