Addgene - Fret-Stop-Fret TOPO Plasmid Data

Plasmid 22774: Fret-Stop-Fret TOPO

Gene/insert name:
fret-STOP-fret
Alt name:
FRT-STOP-FRT
Alt name:
FSF
Insert size:
5600
Vector backbone:
pCR-Blunt II-TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3500
Cloning method:
TOPO Cloning
5' sequencing primer:
M13rev List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
30
Growth instructions:
However, plasmid yield and stability is improved when grown at 30 degrees.
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

Plasmid containing FRT-STOP-FRT cassette for use in expressing genes only after Flp-mediated recombination.

It's like the LSL cassette ( http://www.addgene.org/11584 but this one is a FSF cassette

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras. Jackson et al (Genes Dev. 2001 Dec 15. 15(24):3243-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22774" in your Materials and Methods section.