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Plasmid 22774: Fret-Stop-Fret TOPO
  • fret-STOP-fret

  • FRT-STOP-FRT

  • FSF

  • 5600

  • pCR-Blunt II-TOPO
    (Search Vector Database)

  • Invitrogen

  • Mammalian Expression

  • 3500

  • TOPO Cloning

  • M13rev List of Sequencing Primers

  • Kanamycin

  • DH5alpha

  • 30

  • However, plasmid yield and stability is improved when grown at 30 degrees.

  • Unknown

  • Puromycin

  • View sequences (2)
  • View map

  • Tyler Jacks

  • UBMTA

Comments: 

Plasmid containing FRT-STOP-FRT cassette for use in expressing genes only after Flp-mediated recombination.

It's like the LSL cassette ( http://www.addgene.org/11584 but this one is a FSF cassette

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras. Jackson et al (Genes Dev. 2001 Dec 15. 15(24):3243-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22774" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only