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Plasmid 24284: pMT-Mad2-GFP
  • Mad2

  • CG17498

  • 624

  • D. melanogaster (fly)

  • NM_139734

  • mad2 (Dmel_CG17498, CG17498, Dmel\CG17498, MAD2, Mad2)

  • GFP

  • C terminal on backbone

  • pMT-WG
    (Search Vector Database)

  • Insect Expression

  • 6786

  • AttR1

  • Unknown

  • AttR2

  • Unknown

  • M13 Fwd List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • Ron Vale

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

This cDNA was amplified from a library, ligated into pENTR-D-Topo and then the pENTR insert was ligated into pMT-WG.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. Griffis et al (J Cell Biol. 2007 Jun 18. 177(6):1005-15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24284" in your Materials and Methods section.