Addgene - pGL3-PMT-miR-34a Plasmid Data

Plasmid 25799: pGL3-PMT-miR-34a

Gene/insert name:
hsa-miR-34a promoter
Alt name:
pri-miR34a
Insert size:
2021
GenBank ID:
DB286351
Fusion protein or tag:
luciferase
Terminal:
C terminal on insert
Vector backbone:
pGL3-Basic
(Search Vector Database)
Backbone manufacturer:
promega
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4818
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Judy Lieberman
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

The sequence of the promoter region of miR-34a corresponds to the one described by Raver-Shapira et al. Mol Cell. 2007;26:731–743.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53. Navarro et al (Blood. 2009 Sep 3. 114(10):2181-92. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25799" in your Materials and Methods section.