Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pGL3-PM-34a-K1/2
(Plasmid #25800)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25800 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alternative hsa-miR-34a promoter
  • Alt name
    alternative pri-miR34a transcript
  • Alt name
    pri-miR-34a-K1/2
  • Insert Size (bp)
    2013
  • Tag / Fusion Protein
    • luciferase (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

promoter region of the alternative pri-miR-34a transcript identified by Navarro et al. Blood 2009 Sep 3;114(10):2181-92.

There are several discrepancies between this sequence and the GenBank sequence: T(at position 388) is cahnged to C

"ACACAC" insertion at position 658

"T" insertion at position 1914

A (at position 1599) changed to G

T (at position 1480) changed to G

G (at position 1212) changed to A

T (at position 1199) changed to C

These changes were present in all the clones that the lab sequenced and these clones came from two independent PCRs using genomic DNA extracted from the human erithroleukemia cell line K562. The changes might be due to that particular cell line. Importantly, all the minipreps I tested induced luciferase activity in K562 cells upon TPA treatment (as reported in the manuscript).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-PM-34a-K1/2 was a gift from Judy Lieberman (Addgene plasmid # 25800 ; http://n2t.net/addgene:25800 ; RRID:Addgene_25800)
  • For your References section:

    miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53. Navarro F, Gutman D, Meire E, Caceres M, Rigoutsos I, Bentwich Z, Lieberman J. Blood. 2009 Sep 3. 114(10):2181-92. 10.1182/blood-2009-02-205062 PubMed 19584398