|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24357||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9000
Vector typeInsect Expression
Growth in Bacteria
Insert Size (bp)1500
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer FLP-rec (Common Sequencing Primers)
Mammalian codon-optimized FLP recombinase (FLPo; Addgene plasmid 13792) (Raymond and Soriano, 2007) was PCR amplified to include BglII/NotI restriction sites, and cloned into pQUAST to generate pQUAST-FLPo.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQUAST-FLPo was a gift from Liqun Luo (Addgene plasmid # 24357 ; http://n2t.net/addgene:24357 ; RRID:Addgene_24357)
For your References section:The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990