Addgene - pQUAST-FLPo Plasmid Data

Plasmid 24357: pQUAST-FLPo

Gene/insert name:
FLPo
Insert size:
1500
Vector backbone:
pQUAST
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
9000
Cloning site 5':
BglII
Site destroyed during cloning:
Unknown
Cloning site 3':
NotI
Site destroyed during cloning:
Unknown
5' sequencing primer:
FLP-rec List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (4)
Map:
View map
Supplemental document:
pQUAST-FLPo (GenBank)
Principal Investigator:
Liqun Luo
Terms and Licenses:
MTA

Comments:

Mammalian codon-optimized FLP recombinase (FLPo; Addgene plasmid 13792) (Raymond and Soriano, 2007) was PCR amplified to include BglII/NotI restriction sites, and cloned into pQUAST to generate pQUAST-FLPo.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis Potter et al (Volume 141, Issue 3, 536-548, 30 April 2010 PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24357" in your Materials and Methods section.

Price: $65.00

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