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pCa4B2G-QUAS-DSCP-FLPo
(Plasmid #24359)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24359 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    QUAS-DSCP-FLPo
  • Insert Size (bp)
    2260

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer FLP-rec, pCasper-F
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

FLPo from Addgene plasmid 13792 was PCR amplified to include EcoRI/XbaI restriction sites, and cloned into pQUAS-DSCP to generate pQUAS-DSCP-FLPo. QUAS-DSCP-FLPo-SV40 was excised from pQUAS-DSCP-FLPo by BamHI digestion, and subcloned into pCa4B2G at BamHI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCa4B2G-QUAS-DSCP-FLPo was a gift from Liqun Luo (Addgene plasmid # 24359 ; http://n2t.net/addgene:24359 ; RRID:Addgene_24359)
  • For your References section:

    The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990