|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24704||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerMichael Sofroniew (Addgene Plasmid# 24703)
- Backbone size w/o insert (bp) 18000
Vector typeMammalian Expression
Growth in Bacteria
- Promoter GFAP (glial fibrillary acidic protein)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
A modified Cre (including an artificial intron, nuclear localization signal and HSV-TK polyA was derived from pACN (Bunting et al., (1999) Genes Dev 13: 1524-1528) to create GFAP-Cre as described in Garcia et al., (2004) Nat Neurosci. 7:1233-1241.
Adaptors were used to clone Cre into the NotI/SalI sites of the GFAP-HSV-TK plasmid described in Bush et al. (1998) Cell 93:189-201. Details of the original GFAP (glial fibrillary acidic protein) promoter cassette (GFAP-lacZ) can be found in Johnson et al. (1995) Glia 13:174-184.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GFAP-Cre was a gift from Michael Sofroniew (Addgene plasmid # 24704 ; http://n2t.net/addgene:24704 ; RRID:Addgene_24704)
For your References section:Leukocyte infiltration, neuronal degeneration, and neurite outgrowth after ablation of scar-forming, reactive astrocytes in adult transgenic mice. Bush TG, Puvanachandra N, Horner CH, Polito A, Ostenfeld T, Svendsen CN, Mucke L, Johnson MH, Sofroniew MV. Neuron. 1999 Jun . 23(2):297-308. 10.1016/S0896-6273(00)80781-3 PubMed 10399936