|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24956||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameBNP promoter
SpeciesH. sapiens (human)
Entrez GeneNPPB (a.k.a. BNP)
/ Fusion Protein
- Luciferase (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
An authentic NFAT3 reporter was constructed containing two tandem copies of the sequence 5′-CGCGTCTATCCTTTTGTTTTCCATCCTG-3′ derived from the BNP promoter and previously demonstrated to bind NFAT3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBNP-NFAT-luciferase was a gift from Toren Finkel (Addgene plasmid # 24956 ; http://n2t.net/addgene:24956 ; RRID:Addgene_24956)