Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

9X NFAT-apha-MHC-Luc
(Plasmid #51941)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51941 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL-3 Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 5100
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    9x NFAT binding site
  • Alt name
    Nine copies of an NFAT binding site from the IL-4 promoter (5′-TGGAAAATT-3′)
  • Insert Size (bp)
    81

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    alpha-MHC
  • Alt name
    minimal promoter from the α-myosin heavy chain gene (−164 to +16)
  • Insert Size (bp)
    180
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    9X NFAT-apha-MHC-Luc was a gift from Jeffery Molkentin (Addgene plasmid # 51941 ; http://n2t.net/addgene:51941 ; RRID:Addgene_51941)
  • For your References section:

    Calcineurin/NFAT coupling participates in pathological, but not physiological, cardiac hypertrophy. Wilkins BJ, Dai YS, Bueno OF, Parsons SA, Xu J, Plank DM, Jones F, Kimball TR, Molkentin JD. Circ Res. 2004 Jan 9;94(1):110-8. Epub 2003 Dec 1. 10.1161/01.RES.0000109415.17511.18 PubMed 14656927