pIS1-wt Fzd3 3'UTR
Depositor Sequences: None.
Addgene Sequences: Partial (2)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25024||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerDavid Bartel, Addgene plasmid # 12179
- Backbone size w/o insert (bp) 4085
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namewt Fzd3 3'UTR
Alt namewt Fzd3 reporter
Insert Size (bp)1453
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (unknown if destroyed)
- 3′ cloning site AgeI (unknown if destroyed)
- 5′ sequencing primer Rluc-F (ccaggattcttttccaatgc)
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Renilla luciferase reporter controlled by Fzd3 3'UTR containing wild-type miR-31 binding site motif (TCTTGCCA).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIS1-wt Fzd3 3'UTR was a gift from Bob Weinberg (Addgene plasmid # 25024)
For your References section:A pleiotropically acting microRNA, miR-31, inhibits breast cancer metastasis. Valastyan S, Reinhardt F, Benaich N, Calogrias D, Szasz AM, Wang ZC, Brock JE, Richardson AL, Weinberg RA. Cell. 2009 Jun 12. 137(6):1032-46. 10.1016/j.cell.2009.03.047 PubMed 19524507
Generated by Addgene from plasmid data.