CMV::ratSyGCaMP2
(Plasmid #26124)

• Depositing Lab: Leon Lagnado
• Publication: Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Backbone

• Vector backbone: pEGFP-N1 (Clontech)
• Backbone size w/o insert (bp): 3957
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Synaptophysin GCaMP2
• Alt name: SyGCaMP2
• Species: R. norvegicus (rat)
• Insert Size (bp): 2292
• Entrez Gene: Syp (a.k.a. Syp1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AfeI (not destroyed)
• 3′ cloning site: Ssp1 (destroyed during cloning)
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Reference the following (for GCaMP2) when using this plasmid, along with the original article: Tallini YN, Ohkura M, Choi B-R, Ji J, Imoto K, Doran R, Lee J, Plan P, Wilson J, Xin H-B, Sanbe A, Gulick J, Mathai J, Robbins J, Salama G, Nakai J, Kotlikoff MI (2006) Imaging cellular signals in the heart in vivo: Cardiac expression of the high signal Ca2+ indicator G-CaMP2. Proc. Natl. Acad. Sci. USA 103, 4753-4758
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ORF frame 3 (624-2915): Rat SyGCaMP2.

Our sequence with CMV-F shows a mutation S199T in Synaptophysin ORF. This change does not have any effect in localizing the construct to the synaptic vesicle and may be a result of a SNP.