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CMV::ratSyGCaMP2
(Plasmid #26124)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26124 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1 (Clontech)
  • Backbone size w/o insert (bp) 3957
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synaptophysin GCaMP2
  • Alt name
    SyGCaMP2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2292
  • Entrez Gene
    Syp (a.k.a. Syp1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AfeI (not destroyed)
  • 3′ cloning site Ssp1 (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Reference the following (for GCaMP2) when using this plasmid, along with the original article: Tallini YN, Ohkura M, Choi B-R, Ji J, Imoto K, Doran R, Lee J, Plan P, Wilson J, Xin H-B, Sanbe A, Gulick J, Mathai J, Robbins J, Salama G, Nakai J, Kotlikoff MI (2006) Imaging cellular signals in the heart in vivo: Cardiac expression of the high signal Ca2+ indicator G-CaMP2. Proc. Natl. Acad. Sci. USA 103, 4753-4758
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

ORF frame 3 (624-2915): Rat SyGCaMP2.

Our sequence with CMV-F shows a mutation S199T in Synaptophysin ORF. This change does not have any effect in localizing the construct to the synaptic vesicle and may be a result of a SNP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV::ratSyGCaMP2 was a gift from Leon Lagnado (Addgene plasmid # 26124 ; http://n2t.net/addgene:26124 ; RRID:Addgene_26124)
  • For your References section:

    A genetically encoded reporter of synaptic activity in vivo. Dreosti E, Odermatt B, Dorostkar MM, Lagnado L. Nat Methods. 2009 Dec . 6(12):883-9. 10.1038/nmeth.1399 PubMed 19898484