Addgene - pDRAV-2 Plasmid Data

Plasmid 26747: pDRAV-2

Gene/insert name:
None
Vector backbone:
pBluescript II KS
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Mammalian Expression, Cre/Lox ; FLP/FRT; PhiC31/attB-attP
Backbone size (bp):
3000
Cloning method:
Gateway Cloning
Cloning site 5':
SacI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
T7, M13Fw List of Sequencing Primers
3' sequencing primer:
T3, M13Rv
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Rolf Zeller
Terms and Licenses:
MTA

Comments:

The pDRAV plasmid series encode a 5' loxP site and a 3' FRT site in all possible orientations for dual RMCE (dRMCE) as described in Osterwalder et al. "Dual RMCE for efficient re-engineering of mouse mutant alleles" Nature Methods (2010) DOI 10.1038/nmeth.1521

Protocol for using these plasmids can be found here: http://www.nature.com/protocolexchange/protocol...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Dual RMCE for efficient re-engineering of mouse mutant alleles Osterwalder et al (Nat Methods. 2010 Oct 17 PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26747" in your Materials and Methods section.

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