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dRCME plasmid kit.png

dRMCE Plasmid Kit
(Kit # 1000000015 )

Depositing Labs:   Rolf Zeller

The dual recombinase-mediated cassette exchange (dRMCE) plasmid kit can be used to re-engineer conditional alleles in mouse embryonic stem cells. The dRMCE system takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and can introduce tags, reporters and mutant coding regions into an endogenous locus of interest.

This kit consists of 8 bacterial stabs and will be shipped at room temperature.

Add to Cart
$400 USD + shipping
Available to Academic and Nonprofits Only

Original Publication

Dual RMCE for efficient re-engineering of mouse mutant alleles. Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J. Nat Methods. 2010 Oct 17;7(11):893-5. doi: 10.1038/nmeth.1521. Epub 2010 Oct 17. PubMed

Protocols

For a protocol on how to use these plasmids, see Next generation engineering of conditional mouse alleles with loxP and FRT sites by dual RMCE. Nat Protocol Exchange. Dec 2010. doi:10.1038/protex.2010.210.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The dRCME plasmid kit was a gift from Rolf Zeller (Addgene kit # 1000000015)."

For your Reference section:
Dual RMCE for efficient re-engineering of mouse mutant alleles. Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J. Nat Methods. 2010 Oct 17;7(11):893-5. doi: 10.1038/nmeth.1521. Epub 2010 Oct 17. PubMed

This set consists of these plasmids: