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pDIRE
(Plasmid #26745)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26745 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPGKFlpobpA
  • Backbone manufacturer
    Addgene plasmid 13793
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression, Cre/Lox ; FLP/FRT

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iCre expression cassette
  • Species
    optimized iCre expression cassette; optimized Flpo expression cassette
  • Insert Size (bp)
    2500

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PsiI (destroyed during cloning)
  • 3′ cloning site PsiI (destroyed during cloning)
  • 5′ sequencing primer mPGK-F, EF-1a Forward
  • 3′ sequencing primer BGH-rev, SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid has been assembled using PGK-Flpo cassette (Addgene plasmid 13793), iCre (Addgene plasmid 12336) and Ef1alpha promotor (Addgene plasmid 11918)
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

The pDIRE plasmid is designed to express the optimized versions of the Cre (iCre) and Flp (Flpo) recombinases and is required for dual RMCE (dRMCE) as described in Osterwalder et al. "Dual RMCE for efficient re-engineering of mouse mutant alleles" Nature Methods (2010) DOI 10.1038/nmeth.1521

Protocol for using these plasmids can be found here: http://www.nature.com/protocolexchange/protocols/1906

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDIRE was a gift from Rolf Zeller (Addgene plasmid # 26745 ; http://n2t.net/addgene:26745 ; RRID:Addgene_26745)
  • For your References section:

    Dual RMCE for efficient re-engineering of mouse mutant alleles. Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.. Nat Methods. 2010 Oct 17 10.1038/nmeth.1521 PubMed 20953177