Addgene - pLenti-CaMKIIa-ChETA-EYFP Plasmid Data

Plasmid 26967: pLenti-CaMKIIa-ChETA-EYFP

Purpose:
3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation
Gene/insert name:
hChR2(E123T/H134R)-EYFP
Alt name:
ChETA-EYFP
Insert size:
1662
Species:
H. sapiens (human)
Fusion protein or tag:
EYFP
Terminal:
C terminal on insert
Mutation:
E123T
Vector backbone:
pLenti
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
9250
Cloning site 5':
BamHI, AgeI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
ctcagaagccccaagctcgtc List of Sequencing Primers
3' sequencing primer:
GCAATAGCATGATACAAAGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3 (rec A-) cells to avoid recombinations with the LTRs
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Map:
View map
Supplemental document:
Notes from Addgene (2)
Principal Investigator:
Karl Deisseroth
Terms and Licenses:
UBMTA
Ancillary Agreement for Plasmids Containing FP Materials

Comments:

This plasmid contains the mouse calcium/calmodulin-dependent kinase II alpha subunit promoter.

For additional information please visit - http://www.optogenetics.org

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Ultrafast optogenetic control. Gunaydin et al (Nat Neurosci. 2010 Mar . 13(3):387-92. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26967" in your Materials and Methods section.