PurposeAAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26968||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5587
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth instructionsStbl3 (rec A-) cells to avoid recombinations with the LTRs
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1662
/ Fusion Protein
- EYFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer ggccagcttggcacttgatg
- 3′ sequencing primer GCAATAGCATGATACAAAGG (Common Sequencing Primers)
Terms and Licenses
This plasmid contains the human elongation factor-1a promoter.
For additional information please visit - http://www.optogenetics.org
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-Ef1a-DIO ChETA-EYFP was a gift from Karl Deisseroth (Addgene plasmid # 26968)
For your References section:Ultrafast optogenetic control. Gunaydin LA, Yizhar O, Berndt A, Sohal VS, Deisseroth K, Hegemann P. Nat Neurosci. 2010 Mar . 13(3):387-92. 10.1038/nn.2495 PubMed 20081849
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by Addgene staff.
Map uploaded by the depositor.