|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27059||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7843
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameP#38-mcherry rfp-ssrAec
Insert Size (bp)1280
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGMEH-P38-che-ssrAec3 was a gift from Dirk Schnappinger (Addgene plasmid # 27059)
For your References section:Protein inactivation in mycobacteria by controlled proteolysis and its application to deplete the beta subunit of RNA polymerase. Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D. Nucleic Acids Res. 2010 Nov 12. ():. 10.1093/nar/gkq1149 PubMed 21075796