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pBSDT-AII
(Plasmid #27179)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27179 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Mammalian Expression, Mouse Targeting
  • Selectable markers
    negative selection by diphtheria toxin A

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MC1 promoter diphtheria toxin-A fragment
  • Alt name
    DT-A
  • Species
    synthetic
  • Insert Size (bp)
    1100

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13 forward, T7
  • 3′ sequencing primer M13 reverse, T3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DT-A fragment was obtained from Gibco (discontinued). The original must came from Dr. Shin-ichi Aizawa of RIKEN.
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

The MCS in the pBSDT-AII is extensibly modified by synthetic oligos to create multiple rare restriction sites

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSDT-AII was a gift from Yoh Wada (Addgene plasmid # 27179 ; http://n2t.net/addgene:27179 ; RRID:Addgene_27179)
  • For your References section:

    Simple and straightforward construction of a mouse gene targeting vector using in vitro transposition reactions. Aoyama M, Agari K, Sun-Wada GH, Futai M, Wada Y. Nucleic Acids Res. 2005 . 33(5):e52. 10.1093/nar/gni055 PubMed 15784610