pFC663
(Plasmid
#27324)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27324 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneRS406
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSIC1
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SpeciesS. cerevisiae (budding yeast)
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MutationContains insertion relative to SGDB sequence of GAA to change sequence E129EEEEGE136 to E129EEEEEGE137.
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Entrez GeneSIC1 (a.k.a. YLR079W, BYC1, SDB25)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer M13/pUC-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFC663 was a gift from Fred Cross (Addgene plasmid # 27324 ; http://n2t.net/addgene:27324 ; RRID:Addgene_27324) -
For your References section:
Phosphorylation of the Sic1 inhibitor of B-type cyclins in Saccharomyces cerevisiae is not essential but contributes to cell cycle robustness. Cross FR, Schroeder L, Bean JM. Genetics. 2007 Jul . 176(3):1541-55. 10.1534/genetics.107.073494 PubMed 17483408
