Addgene - pcDNA3 LIC cloning vector (6A) Plasmid Data

Plasmid 30124: pcDNA3 LIC cloning vector (6A)

Gene/insert name:
None
Vector backbone:
pcDNA3
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size (bp):
5407
Cloning site 5':
LIC site
Site destroyed during cloning:
Yes
Cloning site 3':
LIC site
Site destroyed during cloning:
Yes
5' sequencing primer:
CMV F (5'cgcaaatgggcggtaggcgtg) List of Sequencing Primers
3' sequencing primer:
CMV R (5'tggctggcaactagaagg)
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Scott Gradia
Terms and Licenses:
MTA

Comments:

This is a LIC vector derived from pcDNA3. It adds no tags to your protein of interest.

To clone into this vector, add the following tags to your PCR primers:

LIC vKoz Forward tag 5’-TACTTCCAATCCAATGCCACC(ATG)

LicV1 Reverse tag 5’-TTATCCACTTCCAATGTTATTA

T4-treat PCR with dCTP. Linearize vector with SspI, then T4-treat with dGTP. Can verify the presence of insert by digesting with HindIII and XbaI.

For more information, please see our website:
http://qb3.berkeley.edu/qb3/macrolab/

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 30124" in your Materials and Methods section.