Addgene - pcDNA3 mCerulean LIC cloning vector (6E) Plasmid Data

Plasmid 30128: pcDNA3 mCerulean LIC cloning vector (6E)

Gene/insert name:
None
Fusion protein or tag:
mCerulean
Terminal:
C terminal on backbone
Vector backbone:
pcDNA3
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size (bp):
6145
Cloning site 5':
LIC site vKoz/GFP
Site destroyed during cloning:
Yes
Cloning site 3':
LIC site vKoz/GFP
Site destroyed during cloning:
Yes
5' sequencing primer:
CMV F (5'cgcaaatgggcggtaggcgtg) List of Sequencing Primers
3' sequencing primer:
GFP reverse (5'cagctcgaccaggatgggc3')
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Scott Gradia
Terms and Licenses:
MTA

Comments:

This is an empty LIC vector derived from pcDNA3. It adds an mCerulean gene to the C-terminus of your protein of interest.

mCerulean has a excitation max of 435 nm and an emission max of 477 nm.

To clone into this vector, add the following tags to your PCR primers:

LIC vKoz Forward tag 5’-TACTTCCAATCCAATGCCACC(ATG)

LIC vGFP Reverse tag 5’-CTCCCACTACCAATGCC

Do NOT include a stop codon with your reverse primer.

T4-treat PCR with dCTP. Linearize vector with SspI, then T4-treat with dGTP. Can verify the presence of insert by digesting with HindIII and XbaI.

For more information, please see our website:
http://qb3.berkeley.edu/qb3/macrolab/

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 30128" in your Materials and Methods section.