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pcDNA3 mCerulean LIC cloning vector (6E)
(Plasmid #30128)

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Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone size (bp) 6145
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Sequence Information


  • Gene/Insert name
  • Tag / Fusion Protein
    • mCerulean (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site LIC site vKoz/GFP (destroyed during cloning)
  • 3′ cloning site LIC site vKoz/GFP (destroyed during cloning)
  • 5′ sequencing primer CMV F (5'cgcaaatgggcggtaggcgtg)
  • 3′ sequencing primer GFP reverse (5'cagctcgaccaggatgggc3')
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This is an empty LIC vector derived from pcDNA3. It adds an mCerulean gene to the C-terminus of your protein of interest.

mCerulean has a excitation max of 435 nm and an emission max of 477 nm.

To clone into this vector, add the following tags to your PCR primers:



Do NOT include a stop codon with your reverse primer.

T4-treat PCR with dCTP. Linearize vector with SspI, then T4-treat with dGTP. Can verify the presence of insert by digesting with HindIII and XbaI.

For more information, please see our website:

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 mCerulean LIC cloning vector (6E) was a gift from Scott Gradia (Addgene plasmid # 30128)