|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31156||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDavid Turner (University of Michigan Medical Center)
- Backbone size w/o insert (bp) 4595
Vector typeMammalian Expression ; membrane targeted HRP (mHRP)
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1206
Mutationincludes signal sequence and a transmembrane domain before and after the cDNA for HRP
- Promoter CMV promoter including immediate early promoter/enhancer
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
This plasmid expresses EGFP and HRP under the control of two CMV promoters. The membrane-targeted HRP construct (mHRP) was created by adding a signal sequence and transmembrane domain from human CD2 before and after the cDNA of HRP. Please see the associated publication for more information on this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CMV-EGFP-ssHRP-TM was a gift from Hollis Cline (Addgene plasmid # 31156 ; http://n2t.net/addgene:31156 ; RRID:Addgene_31156)
For your References section:Membrane targeted horseradish peroxidase as a marker for correlative fluorescence and electron microscopy studies. Li J, Wang Y, Chiu SL, Cline HT. Front Neural Circuits. 2010 . 4():6. 10.3389/neuro.04.006.2010 PubMed 20204144
Map uploaded by the depositor.