CMV-EGFP-ssHRP-TM
(Plasmid #31156)

• Depositing Lab: Hollis Cline
• Publication: Li et al Front Neural Circuits. 2010 . 4():6.

Backbone

• Vector backbone: BiCS2eGFP
• Backbone manufacturer: David Turner (University of Michigan Medical Center)
• Backbone size w/o insert (bp): 4595
• Vector type: Mammalian Expression ; membrane targeted HRP (mHRP)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ssHRP-TM
• Alt name: mHRP
• Insert Size (bp): 1206
• Mutation: includes signal sequence and a transmembrane domain before and after the cDNA for HRP
• Promoter: CMV promoter including immediate early promoter/enhancer

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid expresses EGFP and HRP under the control of two CMV promoters. The membrane-targeted HRP construct (mHRP) was created by adding a signal sequence and transmembrane domain from human CD2 before and after the cDNA of HRP. Please see the associated publication for more information on this plasmid.