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CMV-EGFP-ssHRP-TM
(Plasmid #31156)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31156 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    BiCS2eGFP
  • Backbone manufacturer
    David Turner (University of Michigan Medical Center)
  • Backbone size w/o insert (bp) 4595
  • Vector type
    Mammalian Expression ; membrane targeted HRP (mHRP)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ssHRP-TM
  • Alt name
    mHRP
  • Insert Size (bp)
    1206
  • Mutation
    includes signal sequence and a transmembrane domain before and after the cDNA for HRP
  • Promoter CMV promoter including immediate early promoter/enhancer

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid expresses EGFP and HRP under the control of two CMV promoters. The membrane-targeted HRP construct (mHRP) was created by adding a signal sequence and transmembrane domain from human CD2 before and after the cDNA of HRP. Please see the associated publication for more information on this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-EGFP-ssHRP-TM was a gift from Hollis Cline (Addgene plasmid # 31156 ; http://n2t.net/addgene:31156 ; RRID:Addgene_31156)
  • For your References section:

    Membrane targeted horseradish peroxidase as a marker for correlative fluorescence and electron microscopy studies. Li J, Wang Y, Chiu SL, Cline HT. Front Neural Circuits. 2010 . 4():6. 10.3389/neuro.04.006.2010 PubMed 20204144