Addgene - pACU2 Plasmid Data

Plasmid 31223: pACU2

Gene/insert name:
None
Vector backbone:
pUAST
(Search Vector Database)
Vector type:
Insect Expression
Backbone size (bp):
9367
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
TCTTACTGACATCCACTTTGCC List of Sequencing Primers
3' sequencing primer:
GGCGCACAGAAATGATTACAAC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Yuh-Nung Jan
Terms and Licenses:
MTA

Comments:

A modified pUAST vector containing both P-elements and attB site for high expression of UAS-driven transgenes

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Enhancer-driven membrane markers for analysis of nonautonomous mechanisms reveal neuron-glia interactions in Drosophila. Han et al (Proc Natl Acad Sci U S A. 2011 May 23. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31223" in your Materials and Methods section.