pT-FLAG
(Plasmid #31385)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    phrGFP
  • Backbone manufacturer
    Stratagene
  • Backbone size (bp) 3501
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT-FLAG was a gift from Yegor Vassetzky (Addgene plasmid # 31385)
  • For your References section:

    A set of vectors for introduction of antibiotic resistance genes by in vitro Cre-mediated recombination. Dmitriev PV, Vassetzky YS. BMC Res Notes. 2008 . 1():135. 10.1186/1756-0500-1-135 PubMed 19105832