Addgene - pT-FLAG Plasmid Data

Plasmid 31385: pT-FLAG

Gene/insert name:
None
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Vector backbone:
phrGFP
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Mammalian Expression
Backbone size (bp):
3501
Cloning site 5':
NheI
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
Yes
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Yegor Vassetzky
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A set of vectors for introduction of antibiotic resistance genes by in vitro Cre-mediated recombination. Dmitriev et al (BMC Res Notes. 2008 . 1():135. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31385" in your Materials and Methods section.