Addgene - pLKO.1-GSK3β-#2 Plasmid Data

Plasmid 32497: pLKO.1-GSK3β-#2

Gene/insert name:
GSK3B
shRNA sequence:
(AAA)GCTAGATCACTGTAACA
Species:
H. sapiens (human)
Entrez Gene:
GSK3B ()
Vector backbone:
pLKO.1
(Search Vector Database)
Backbone manufacturer:
Sabatini Lab
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7000
Promoter:
U6
Cloning site 5':
None
Site destroyed during cloning:
Unknown
Cloning site 3':
None
Site destroyed during cloning:
Unknown
5' sequencing primer:
LKO.1 (GACTATCATATGCTTACCGT) List of Sequencing Primers
3' sequencing primer:
none
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Alex Toker
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Akt/protein kinase b and glycogen synthase kinase-3beta signaling pathway regulates cell migration through the NFAT1 transcription factor. Yoeli-Lerner et al (Mol Cancer Res. 2009 Mar;7(3):425-32. Epub 2009 Mar 3. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32497" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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32496: pLKO.1-GSK3β-#1