Addgene - pTYF-G4BS-3.6TPH-EGFP Plasmid Data

Plasmid 32570: pTYF-G4BS-3.6TPH-EGFP

Gene/insert name:
EGFP
Insert size:
798
Species:
jellyfish
Vector backbone:
pTYF
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
7469
Promoter:
3.6TPH
Cloning site 5':
SpeI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
atggtgagcaagggcgaggag List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
30
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Sergey Kasparov
Terms and Licenses:
MTA

Comments:

Please note that Addgene's quality control sequence shows A inserted relative to the author's sequence. There is no evidence that this insertion affects the function of the plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Targeting central serotonergic neurons with lentiviral vectors based on a transcriptional amplification strategy. Benzekhroufa et al (Gene Ther. 2009 May;16(5):681-8. Epub 2009 Feb 12. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32570" in your Materials and Methods section.