Mef2A-shRNA
(Plasmid
#32899)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32899 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSuper
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Backbone manufacturerOligoEngine
- Backbone size w/o insert (bp) 3176
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Vector typeMammalian Expression, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMef2a
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gRNA/shRNA sequence5’-GTTATCTCAGGGTTCAAAT-3’
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SpeciesR. norvegicus (rat)
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Entrez GeneMef2a
- Promoter H1-RNA
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site none (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Can be used in conjunction with pcDNA3-rMEF2A (Addgene #32901) which is mutated so as to not be targeted by this shRNA.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mef2A-shRNA was a gift from Michael Greenberg (Addgene plasmid # 32899 ; http://n2t.net/addgene:32899 ; RRID:Addgene_32899) -
For your References section:
Activity-dependent regulation of MEF2 transcription factors suppresses excitatory synapse number. Flavell SW, Cowan CW, Kim TK, Greer PL, Lin Y, Paradis S, Griffith EC, Hu LS, Chen C, Greenberg ME. Science. 2006 Feb 17;311(5763):1008-12. 10.1126/science.1122511 PubMed 16484497