Addgene - pBabe puro-HA-PIK3CA(H1047R;G320E) Plasmid Data

Plasmid 33254: pBabe puro-HA-PIK3CA(H1047R;G320E)

Gene/insert name:
PIK3CA
Insert size:
3200
Species:
H. sapiens (human)
Entrez Gene:
PIK3CA (CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha)
Fusion protein or tag:
Hemagglutinin (HA)
Terminal:
N terminal on insert
Mutation:
changed histidine 1047 to arginine and glycine 320 to glutamic acid
Vector backbone:
pBabe puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5168
Promoter:
SV40
Cloning method:
Ligation Independent Cloning
5' sequencing primer:
CTTTATCCAGCCCTCAC List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
from JJ Zhao, DFCI, Boston MA.
Sequence: View sequences (5)
Principal Investigator:
Thomas Roberts
Terms and Licenses:
MTA

Comments:

Note from the depositing lab: "This plasmid contains an additional mutation, I143V, that is not thought to alter function of the protein"

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: PI3K-targeted therapy can be evaded by gene amplification along the MYC-eukaryotic translation initiation factor 4E (eIF4E) axis. Ilic et al (Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):E699-708. Epub 2011 Aug 29. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33254" in your Materials and Methods section.