Addgene - pJMK004 Plasmid Data

Plasmid 34616: pJMK004

Gene/insert name:
Arch D95N
Alt name:
Archaerhodopsin 3
Insert size:
777
Species:
Halorubrum sodomense
Fusion protein or tag:
GFP
Terminal:
C terminal on insert
Mutation:
Changed Aspartic Acid 95 to Asparagine
Vector backbone:
FCK(1.3)
(Search Vector Database)
Backbone manufacturer:
Pavel Osten
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
9227
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
GACGGATCCGCCACCATGGAC List of Sequencing Primers
3' sequencing primer:
CTAATCGAATTCTTAGTCGGCGGCAC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Adam Cohen
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Optical recording of action potentials in mammalian neurons using a microbial rhodopsin. Kralj et al (Nat Methods. 2011 Nov 27. doi: 10.1038/nmeth.1782. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34616" in your Materials and Methods section.

Price: $65.00

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