|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34893||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAoki et al., PNAS. 1998 Dec 8;95(25):14950-5
- Backbone size w/o insert (bp) 2949
Vector typeAdaptor plasmid
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationSee depositor comments below.
Entrez GenePIK3CD (a.k.a. APDS, IMD14, P110DELTA, PI3K, p110D)
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Addgene NGS results found a P2A variant compared to the NCBI reference [NP_005017.3].
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBSFI-p110δ was a gift from Peter Vogt (Addgene plasmid # 34893 ; http://n2t.net/addgene:34893 ; RRID:Addgene_34893)
For your References section:Oncogenic transformation induced by the p110beta, -gamma, and -delta isoforms of class I phosphoinositide 3-kinase. Kang S, Denley A, Vanhaesebroeck B, Vogt PK. Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1289-94. Epub 2006 Jan 23. 10.1073/pnas.0510772103 PubMed 16432180