1
Plasmid 20573: pWZL Neo Myr Flag PIK3CB
  • PIK3CB

  • phosphoinositide-3-kinase, catalytic, beta polypeptide

  • H. sapiens (human)

  • NM_006219

  • PIK3CB (P110BETA, PI3K, PI3KBETA, PIK3C1)

  • Myr

  • N terminal on backbone

  • Flag

  • N terminal on backbone

  • pWZL-Neo-Myr-Flag-DEST
    (Search Vector Database)

  • William Hahn Lab (available at Addgene, #15300)

  • Mammalian Expression, Retroviral

  • Gateway Cloning

  • pWZL-Fwd (GAA CCT CCT CGT TCG ACC) List of Sequencing Primers

  • pWZL-Rev (TTC CGG GCC CTC ACA TTG)

  • Ampicillin

  • Stbl3

  • 37

  • Stbl3 cells to prevent recombination.

  • Unknown

  • Neomycin

  • 2-G11

  • View sequences (1)
  • William Hahn

  • Jean Zhao

  • MTA

Comments: 

ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.

This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene (Kinase Library Clones) Boehm et al (Cell. 2007 Jun 15. 129(6):1065-1079. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20573" in your Materials and Methods section.