Addgene - PIK3CA-WT Plasmid Data

Plasmid 16643: PIK3CA-WT

Gene/insert name:
PIK3CA
Alt name:
Phosphatidylinositol 3-kinase
Alt name:
p110 alpha
Species:
H. sapiens (human)
Entrez Gene:
PIK3CA (CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Vector backbone:
pCMV2-Tag 2A
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4300
Cloning site 5':
EcoRV / XmnI
Site destroyed during cloning:
Yes
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T3 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Bert Vogelstein
Terms and Licenses:
MTA

Comments:

This plasmid has 2 mutations in the ORF, N115D and L178S. These mutations do not alter the function of the encoded proteins.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: High frequency of mutations of the PIK3CA gene in human cancers. Samuels et al (Science. 2004 Apr 23. 304(5670):554. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16643" in your Materials and Methods section.