Addgene - pLenti-CaMKIIa-C1C2-TS-EYFP Plasmid Data

Plasmid 35520: pLenti-CaMKIIa-C1C2-TS-EYFP

Gene/insert name:
ChR1-ChR2 Chimera
Alt name:
C1C2
Insert size:
1830
Species:
Chlamydomonas reinhardtii
Fusion protein or tag:
Trafficking signal and EYFP
Terminal:
C terminal on insert
Vector backbone:
pLenti-CaMKIIa
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
9226
Modifications to Backbone:
Has a CaMKIIa promoter and a WPRE
Promoter:
CaMKIIa
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
AGTCCTGCAGTATTGTGTAT List of Sequencing Primers
3' sequencing primer:
GCAATAGCATGATACAAAGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Karl Deisseroth
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Crystal structure of the channelrhodopsin light-gated cation channel. Kato et al (Nature. 2012 Jan 22. doi: 10.1038/nature10870. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35520" in your Materials and Methods section.