|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35524||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeYeast Expression, Synthetic Biology
Growth in Bacteria
Gene/Insert nameBisabolene Synthase
- Promoter P GAL 1
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (unknown if destroyed)
- 3′ cloning site Xho1 (unknown if destroyed)
- 5′ sequencing primer agttctagaatccggggt
- 3′ sequencing primer CATGTAATTAGTTATG (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRSLeu2d-BISopt was a gift from Taek Soon Lee (Addgene plasmid # 35524 ; http://n2t.net/addgene:35524 ; RRID:Addgene_35524)
For your References section:Identification and microbial production of a terpene-based advanced biofuel. Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, Lee TS. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. 10.1038/ncomms1494 PubMed 21952217