pg50-2.11 (#65)
(Plasmid #35966)

• Depositing Lab: Thomas Sato
• Publication: Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3058-63.

Backbone

• Vector backbone: pBluescript II SK (+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Growth instructions: This plasmid is not very stable (the bacteria lose or even rearrange it quite often). Pool miniprep-DNA from freshly transformed bacteria instead of midi- or maxiprep.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Tie2 promoter-1st exon-1st intron
• Species: M. musculus (mouse)
• Insert Size (bp): 17000

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TIE2 genomic subclone DNA containing the 10kb enhancer fragment (NaeI-SalI or NotI)

The depositing laboratory recommends digesting plasmid DNA obtained by minipreps with NaeI+SalI or NotI to verify the presence of the 10 kb enhancer fragment.