Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36316||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7000
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameZifCY2 genetically encoded Zn(II) sensor
Alt nameHis4 sensor
Insert Size (bp)1515
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
First generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from mammalian Zif268. ZifCY2 is a variant of ZifCY1. The two cysteines (amino acids 237 and 242) in the Zn(II)-binding domain of ZifCY1 are mutated to histidines in ZifCY2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA-ZifCY2 was a gift from Amy Palmer (Addgene plasmid # 36316 ; http://n2t.net/addgene:36316 ; RRID:Addgene_36316)
For your References section:Genetically encoded sensors to elucidate spatial distribution of cellular zinc. Dittmer PJ, Miranda JG, Gorski JA, Palmer AE. J Biol Chem. 2009 Jun 12;284(24):16289-97. Epub 2009 Apr 10. 10.1074/jbc.M900501200 PubMed 19363034
Map uploaded by the depositor.