Addgene - pJFRC81-10XUAS-IVS-Syn21-GFP-p10 Plasmid Data

Plasmid 36432: pJFRC81-10XUAS-IVS-Syn21-GFP-p10

Gene/insert name:
GFP
Insert size:
717
Vector backbone:
pJFRC-MUH
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
8508
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
hsp70 F: GAGCGCCGGAGTATAAATAGAG List of Sequencing Primers
3' sequencing primer:
p10 R: CGGCCAAATGTTAAACTTGGAG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Gerald Rubin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Using translational enhancers to increase transgene expression in Drosophila. Pfeiffer et al (Proc Natl Acad Sci U S A. 2012 Apr 9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36432" in your Materials and Methods section.