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Cre Stoplight 2.4
(Plasmid #37402)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37402 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4273
  • Total vector size (bp) 5241
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cre Stoplight 2.4
  • Alt name
    Stoplight
  • Insert Size (bp)
    2023
  • Promoter CMV
  • Tags / Fusion Proteins
    • ZsGreen1 (N terminal on insert)
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1, EcoRV etc. (unknown if destroyed)
  • 3′ cloning site loxp (unknown if destroyed)
  • 5′ sequencing primer CAG enhancer
  • 3′ sequencing primer Amp
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note that Cre Stoplight 2.4 differs from the original Cre Stoplight described in the associated publication (Biotechniques. 2001 Nov;31(5):1036, 1038, 1040-1.). See the supplemental document Cre Stoplight 2.4 above for more information.

Please note that Addgene digest and NGS results suggest that the plasmid contains two mCherry ORFs in tandem. This is not expected to affect plasmid function as the second mCherry should not be expressed from the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cre Stoplight 2.4 was a gift from Thomas Hughes (Addgene plasmid # 37402 ; http://n2t.net/addgene:37402 ; RRID:Addgene_37402)
  • For your References section:

    Cre stoplight: a red/green fluorescent reporter of Cre recombinase expression in living cells. Yang YS, Hughes TE. Biotechniques. 2001 Nov;31(5):1036, 1038, 1040-1. 10.2144/01315st03 PubMed 11730010